Sequences from confirmation amplicon


Confirmation sequences for line SALK_151939 and hit At1g53850 are shown.
Line ID SALK_151939
Locus At1g53850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20104861
Primers used for PCR 67cs - LBb3, show primer details
   
Sequence Name 93-K109033-SALK_151939C-67cs
GenBank Accession LN486351 [GenBank]
Sequencing primer gene specific primer, 67cs: 5'-AAAAGGGATTGTTGAATTTTGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K109033-SALK_151939C-67cs
GTTCTCTGCAATTGGAGTAAGACAAAGAAGGAGTTGTGCTCGTTAACGAGAAGCGTATCA
CGTCGCCATTGCTGGTTTGCTTGCTAACTTTGAATTCTATGTTTTTCAGAATTTTTCTTG
TGATTCACTATTTTGGAACAATTTGAATTGAATCTGATGATATGATCAAGCAGAAGCATA
TAAGCTTTCTATGTTAGTTATGGTGATATTAGCTTTCTCAGTATATGTTAAATGCTTTTT
CTTTTTCCCCAGGAACCGAGCAGTGTGGAGAAGATTATGGAAATTGATGACCATATTGGT
TGTGCTATGAGCGGTTTAATTGCTGACGCGCGCACACTTGTTGAGCATGCTAGAGTTGAA
ACTCAAGTAAGGTTTCTGTCTCTAGGGTCTTTTCTTTTAGCTTCAACAGGTCTTTTATGT
GTTATTGACGGCTTCTTTTGCTCTTGTACAGAACCATAGATTCTCGTATGGTGAGCCGAT
GACTGTAGAGTCCACAACACAAGCACTGTGTGATCAGGATATATTGTGGTGTAAACAAAT
TGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTT
CTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGT
TGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGGTTGATGGTGGTT
CCGAAA
   
Sequence Name 77-K109032-SALK_151939C-R210
GenBank Accession LN486168 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K109032-SALK_151939C-R210
GTCATTTGTTTACCCACAATATATCCTGATCACACAGTGCTTGTGTTGTGGACTCTACAG
TCATCGGCTCACCATACGAGAATCTATGGTTCTGTACAAGAGCAAAAGAAGCCGTCAATA
ACACATAAAAGACCTGTTGAAGCTAAAAGAAAAGACCCTAGAGACAGAAACCTTACTTGA
GTTTCAACTCTAGCATGCTCAACAAGTGTGCGCGCGTCAGCAATTAAACCGCTCATAGCA
CAACCAATATGGTCATCAATTTCCATAATCTTCTCCACACTGCTCGGTTCCTGGGGAAAA
AGAAAAAGCATTTAACATATACTGAGAAAGCTAATATCACCATAACTAACATAGAAAGCT
TATATGCTTCTGCTTGATCATATCATCAGATTCAATTCAAATTGTTCCAAAATAGTGAAT
CACAAGAAAAATTCTGAAAAACATAGAATTCAAAGTTAGCAAGCAAACCAGCAATGGCGA
CGTGATACGCTTCTCGACAGCAAGCACAACTCCTTCTTTTGTCTTTACTCCAATTGCAGT
AGAACCAAGCTGCAACAAAATTCAACACTCATCAGAGTCTCAACAAAATTCAACAATCCC
TTTTA

Last Updated on 06.12.2018 16:11