SimpleSearch - Line and FST details
Line specific information
| Line ID | 013E09 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401209) |
| Segregation Analysis | 100:56:44 |
| Confirmed for Hit | At1g05570 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit F22C12
| Sequence (A. th genome BLAST matches underlined) | >57-K011902-0022-013-E09-8409 GTGAACCATGTAGTATTTCCCGGCTACATGAAGCCATCTACCTATGAATCTAGGGCCGGT GTCCGACTATTCGTGGATCCCTATCACTAATAGCATAGACAAGCACTCACATAAAGAAAG CTTATTAGAGCGATCAAAGACAAACTTTATTACTCTCAGTTTGCATAAGCTGTTTCTCAT ACTCAAAATCATGGGATATTCTTTGATCTGTTTAAACACTTGCGTGGCCTCCATAGGAGG GATTAAACTATTTATAGTTAAGTATGCACCACTAAATAACATTTAATGTATGGATCAGAA ACTCTGGTTAAGGAGATAAAGTTGCCTAAGGAGAGGACTAAAAGTCTTTATGTCTCCAAA GGATGATTGTGAACACTTTCGTAGGTCGTTACCTATGGGATCCTCCCTATAGTGAGTCGA TNTACTC |
| GenBank Accession | FR800301 [GenBank] |
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| Predicted Position of Insertion | Chr1:23750449 - go to primer design |
| BLAST e Value | 4e-78 |
| Hit Clone Code (BAC ID) | F22C12 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | FR800285 [GenBank] |
Gene hit MMI9
| Sequence (A. th genome BLAST matches underlined) | >57-K011967-0022-016-E09-8409 ACAGAGAGAACTATTACTTGATTCGGATCGATCCTGGATTCTGGACCATCTTTGGTGGAT GCATATCAACTGTAATGACATTGAATTTTACTCGAGGAGTGCCTTGGAATAATAACTAAT TCAGTAGCAGCTCGCAACCACCGTTAATTGTATGGGCCAAAGGCTATGAGTATATTATGA TTGATTTGACGGACAAGCTTTCACGAAAGTGAAGACTATGGGATCCTCCCTATAGTGAGT CGANTTACTC |
| GenBank Accession | HE664184 [GenBank] |
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| Predicted Position of Insertion | Chr5:24991218 - go to primer design |
| BLAST e Value | 3e-51 |
| Hit Clone Code (BAC ID) | MMI9 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At1g01120
| Sequence (A. th genome BLAST matches underlined) | >69-K012791-022-013-E09-35St AGACCCTTCCTCTATATAAGGAATGTACTTTCATTTGGAGAGGACAGGGTCCCCGGGGAT CAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATAAATGTTTGAATGATCCATGGTGCTG AACCATCGGCTAGAACGAATCTACTGATATATTACGCTCGTCTTCC |
| GenBank Accession | AL752489 [GenBank] |
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| Predicted Position of Insertion | Chr1:58485 - go to primer design |
| BLAST e Value | 3e-08 |
| Hit Clone Code (BAC ID) | T25K16 |
| Hit Gene Code | At1g01120 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | 3-ketoacyl-CoA synthase 1 |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g05570
| Sequence (A. th genome BLAST matches underlined) | >69-K025793-022-013-E09-8409 TATATATCACTGAATAGTTTACCGAAGGCGGGAAACGACAATCTGATCCCCGGGATATGG GATCCTCCCTATAGTGAGAGNANANCANACACATAAAGAAAGTTATAAAGGGACCAAAGA CAAAATTTATTACTCTCCGCTTGTAAAAGCTGCTCCTCAGACCAAATCTGTGGATTCTTC TGATTCCGTTCCAAAAATACATCAGGTATAAGGTGAGAAAACTAAGTAAATATACTTAAC ATTTTGGTGCTCCTTTTTTTCCCAAAGTACGAAGATGAGTTTCTGCCTATGCAGAGAGGT CTGACGATATAGATGCTGCTGTTCCTTAGAGACTGAGTTATATTTATTTTCTGTCTTCTC ATTTATCGGATTAAGCTTCCGGGACACCCTATACTAGGAGAGGCAACGCCGGAAAATCAG AATCATGCTATCTTATTTACCCGTGGAGAAGGTTTGCAGATAATAGACGTGAATC |
| GenBank Accession | CR394982 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:1650884 - go to primer design |
| BLAST e Value | 2e-103 |
| Hit Clone Code (BAC ID) | T25N20 |
| Hit Gene Code | At1g05570 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | callose synthase 1 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | CR395377 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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