SimpleSearch - Line and FST details
Line specific information
| Line ID | 019A12 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401740) |
| Segregation Analysis | 50:43:37 |
| Confirmed for Hit | At3g62260 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit At3g22520
| Sequence (A. th genome BLAST matches underlined) | >89-K013477-022-019-A12-8409 ATTGGGCCGATCATATTAATTTCTAAACAGATATGGCGCCTAGATGAGCTACAACAACAC ATTTAGGTAGATAAAATGATGCCAAAAGCTCAATTTAAACGCAAACTCTGGGATCCTCCC TATAGTGAGAGGGATATCCTGAGTCTGAATCTTTGCTTTGATTAATTGAACAACTTAGAC CTTTTGATGGGTTTATGTCCGGAAATGACACCAAATTAGGGTTTCGCTGATATCTGCGCA AAATTCTGACCTAAGCACAAAAAGTTCTTGATTTTAGATGAGAGAAAATTAATTTTGTCG AGAGGAACATTCCTGTCCTTTGGGAATTGTGTGAAACTTACCGTGAGCACAAGTTAGGGC TCCTATTCTCACTCGCTGCTACCAATAAAGGATGTGCCCTTTAGGGGTTGACGCCCCCAT GATCCGCGTATCATTATGCCCTGACTCCGCCTAAGTCAAACACTCCGGGCAAATGGAAGC GGGCAAAAAAATTGTTCGGGCCCACTTCTAATACCCGCGGCTTCCCGAAAAGGCCAAACG CATCTCGGCGCTGTGGTTAGGTTCGGGCCAACCCACCTTCCTCCTTGGCTTCCCCATGGG GATCCACCCTCCACAGGGCACCACCACACAAATATCCTCCCCACATTCCCTTTCCTCCCA AACATTTCCGTCATATAACCCGTTTCCCTAACATCACAATTCCCATTTTT |
| GenBank Accession | AL753033 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:7974813 - go to primer design |
| BLAST e Value | 2e-74 |
| Hit Clone Code (BAC ID) | F16J14 |
| Hit Gene Code | At3g22520 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | spindle assembly abnormal protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 017E03 |
Gene hit At2g23780
| Sequence (A. th genome BLAST matches underlined) | >89-K013160-022-019-A12-8409 NATTGATCCATGTAGATCTTCCCTTACATGAANGCCTTTACACTTGAATATATCCTGTAG ATCCCTATTAAGTAGAGATTTGGACGAAACAAAAGAGGAAACCAGACATCTTGGGAAAAA ACAGAGTCTTACTGTTGTTGGGAAAGAAAGTTCCAGGCTATATCTGACTTTTGGTTTTTC TCGTTTTTTTTCCATATATTTTCTTTGGAAGTTTTGATTAATTATTGGGCCGATTATATT AATTTCTAAACTGATATTGGGCCTGATTAGCTACAACAACACATTTTGGTAGATAAAATG ATGCCAAAAGCTCGTTTTAAACGTAAACTATGGGATCCTCCCTATAGTGAGTCGTATTAC TC |
| GenBank Accession | AL753032 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:10124534 - go to primer design |
| BLAST e Value | 5e-133 |
| Hit Clone Code (BAC ID) | F27L4 |
| Hit Gene Code | At2g23780 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RING/U-box superfamily protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | unknown |
Gene hit At3g62260
| Sequence (A. th genome BLAST matches underlined) | >71-K017011-022-019-A12T-8409 GATCTGTAGATTCCNGGACATGAGCCATTTACAATTGATATATCCTGCGCATAAACACAA GATCTGACAGAGCGGTGGTACCACAAGATCGCTGATACTGCAATCCTCTGCCAAAGCTAT GGGATCTCNCCTATAGTGAG |
| GenBank Accession | AL936105 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:23039359 - go to primer design |
| BLAST e Value | 1e-16 |
| Hit Clone Code (BAC ID) | T17J13 |
| Hit Gene Code | At3g62260 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Protein phosphatase 2C family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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