SimpleSearch - Line and FST details
Line specific information
| Line ID | 038F12 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N403624) |
| Segregation Analysis | 50:47:41 |
| Confirmed for Hit | MQJ2 At5g33290 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 85568, 85573, 85583 |
Gene hit At5g33300
| Sequence (A. th genome BLAST matches underlined) | >94-K015223-022-038-F12-8409 NTGATCCATGTAGATTNTCCCGGACATGAANGCCTTTACAATAGCTTATATTTCTTTCTT TCTATTGCTTACTCTGAAACTTGGATGCGATAGAGGATAGATCAAATTAATGTATACTGT ATATACTCTATAGGGCATTTTGGCATACTAATTCTATTTAATTATAAACTGTTGTGCGAC TCGCCTCGAATAGGATTATATGAGTTTTCGTTCGACAGATGCGATACTCAGCACGCGCCA CTGCTATGGGATCCTCCCTTCATTGAGTTTGGCGTTCGTAATCAGAATGAATTTTATTCA GTTTCATTAGTAGGCCTGATTCATTGCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | AL763635 [GenBank] |
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| Predicted Position of Insertion | Chr5:12563443 - go to primer design |
| BLAST e Value | 8e-27 |
| Hit Clone Code (BAC ID) | F19N2 |
| Hit Gene Code | At5g33300 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | chromosome-associated kinesin-like protein |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At2g44340
| Sequence (A. th genome BLAST matches underlined) | >94-K030267-022-038-F12-8409 TGAAGCCTTTACAACTTGAAGATATTGACGACTTCCAAATTCCACCTTGTGATTCTTCTG TCTTCTCCCAGAAATAACTCTTCCTCACGGTATCCTCCCTATATTGAGTCCTAATACTCG TGCTCATTTCCGAATACCCTTCTCCCTATCGTGAGTCACATTCCGCATTTACATTTTCAA CTTCCCTTACTTATTATCCCCCCAACATACCACCTTATCGTTGCAATTACCTCACATCTC ACCAAATGCCATTTGCCNAATCAATAATATTGAAAACCCTANAAATTACTCTGTTATTAA TTAAAACCAATTATGAGCCTTTGTATCGAAGTTGGTGTT |
| GenBank Accession | CR934234 [GenBank] |
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| Predicted Position of Insertion | Chr2:18315108 - go to primer design |
| BLAST e Value | 5e-39 |
| Hit Clone Code (BAC ID) | F4I1 |
| Hit Gene Code | At2g44340 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | VQ motif-containing protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | unknown |
Gene hit MQJ2
| Sequence (A. th genome BLAST matches underlined) | >94-K015223-022-038-F12-8409 NTGATCCATGTAGATTNTCCCGGACATGAANGCCTTTACAATAGCTTATATTTCTTTCTT TCTATTGCTTACTCTGAAACTTGGATGCGATAGAGGATAGATCAAATTAATGTATACTGT ATATACTCTATAGGGCATTTTGGCATACTAATTCTATTTAATTATAAACTGTTGTGCGAC TCGCCTCGAATAGGATTATATGAGTTTTCGTTCGACAGATGCGATACTCAGCACGCGCCA CTGCTATGGGATCCTCCCTTCATTGAGTTTGGCGTTCGTAATCAGAATGAATTTTATTCA GTTTCATTAGTAGGCCTGATTCATTGCTATGGGATCCTCCCTATAGTGAG |
| GenBank Accession | AL763635 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:23636680 - go to primer design |
| BLAST e Value | 1e-13 |
| Hit Clone Code (BAC ID) | MQJ2 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g33290
| Sequence (A. th genome BLAST matches underlined) | >94-K015215-022-038-F12-8409 NTTTGATCCATGTAGATTTCCCTTGACTGAANGCCTTTACAATTGAATATATCCTGCCGC CGCTGCTGTTTTGCACCCGGTGGAGCTTGCATGTTGGTTTAAAATTGTCAGCGCTATCAT ATTTGACTAAAAATCTAGGAACTGTCAAATACTGCACTATGGGATCCTCCCTATAGTGAG ANCTANANTGTTNGTCTAAAACTGTATGAATTGTCATATATTTTAGTAGGGGATCCCTCT GTTATAGAGACTATCTCGAGTTCTTTGTTCATAATCTCACTGAATTTTCTTCAATTC |
| GenBank Accession | AL763634 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:12559199 - go to primer design |
| BLAST e Value | 1e-22 |
| Hit Clone Code (BAC ID) | T29A4 |
| Hit Gene Code | At5g33290 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | xylogalacturonan deficient 1 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At4g38760
| Sequence (A. th genome BLAST matches underlined) | >94-K030252-022-038-F12-8409 TGGCATCCTCCCTATAGTGAGTCATATTACTCCCTTACATTTTAGTCTTCCCCTACTAAA GACTCGACTGCTGAACGNAAGGATAAGAAAAACTCCCNAAATTGTTAGTGAAATATCANT GTCAGGCTTCTGTTTTGGANATTATGGNTTGCNACATGNTTTTGNANGAGATCCGCCCAA TAAAGAGAGGAACGAGCGNNAACCACACTTNAGTGACAGTGTCTCATATAGGGAGGCTGC AAGACGGCATCGAAGAAACCAGGGGCGCCATGGAGGAAGGTCGGGATACACCAGCGGCGG CCCGAGGGAGCAACTACTTATATCGCCCACCGCGGCACCAAGAAACTCAGCGCCGCGAGA TAAAAATAACCACATAC |
| GenBank Accession | CR934233 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr4:18089072 - go to primer design |
| BLAST e Value | 2e-20 |
| Hit Clone Code (BAC ID) | T9A14 |
| Hit Gene Code | At4g38760 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | nucleoporin (DUF3414) |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
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Last Updated on 10.06.2021 13:37 |
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