SimpleSearch - Line and FST details
Line specific information
| Line ID | 052G05 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N404973) |
| Segregation Analysis | 100:99:74 |
| Confirmed for Hit | At5g50590 At3g29725 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 65522, 95704, 95705 |
| Note | |
Gene hit At5g49980
| Sequence (A. th genome BLAST matches underlined) | >39-K012299-022-052-G05-35St TTGACGAAGGGATGACGCACAATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GTTTAAACTATCAGTGAACTTCCCTGAACTTCAGTTATGCTAACATTTCTCCTGACATGT TCAAGCCCATCATACTCAATTGCCACAAACTCCAGGTGTTCTGGGCATGTATCCTTCTTG ATCTATGTCTTCATGGCTTTATTAAGTTATATGTGACTTAACTCTATGGGATCCTCCCTA TAGTGAGTCGCATTACTAAGTCGGAAAGCCTGACGACGGAGCAGCATTCCAAAAAGATCC CTTGGCTCGTCTGGGTCGGCTATGGGATCCTCCCTATAGGGAGTCTTATTACT |
| GenBank Accession | AL753846 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:20335424 - go to primer design |
| BLAST e Value | 2e-74 |
| Hit Clone Code (BAC ID) | K9P8 |
| Hit Gene Code | At5g49980 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | auxin F-box protein 5 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 051G05 |
Gene hit At5g50590
| Sequence (A. th genome BLAST matches underlined) | >39-K012482-022-052-G05-8409 TGATCCATGTAGACTTTCCCTGACATGAAGCACTTTACACTTGAATATATCCTGATGAGC TGAAGATGTCAAACTCCGAGCTCGGCCGCATCGCCATATCTACAGCCGCCGTCAGCGATT TCCTTGGATTTCTCACCCTATGGGATCCTCCCTATAGTGAGAATGAATGATTNGCTTCAC GCAAGCAAAGCAGCTATGATAAACTTGTACGACACTCTTAGGATCCATCTTCATGCAGAA GAAGGAGAGACAACTCGGGTTTCTTGGCCTAATCGAAAACGGAAATACTAATCCGGACCT TTTAGATGAGGGATTGTACTATCATCATTTTTCATTTATAGTCTGTGCAAGTGTTATGAC TGAAGTATCTTTTGCAGAAACAAGACTGGTCGCAAGTTGTGACCGTCAAGTCAGCAGCGG AATGCTCCAAAGCGGTAGCTAACGGAATCTGCACAGGCAAAACCTTTGCGGCAGAGCCGC AGTGGGTTCTTGACCTGTACTGGCTTACTGCTATATGCCCTGAGCTCTTGAACTGTAACC CTAAAAAGAGTTGACCAGAGGTGTTGTTAAATAATTGCATAACAGAGATAAATATGTGAA ATACAACATGATGGCCATCTAGAAGTTTAGGCTAAATATGCTTATTTTAAGAGGTCAACC CAAGCTTTCTTTTAGAGGAAGGGACTCTGGGAAATTTTAAACTGTGTGAAATGCCGTTAG AATATGGAAA |
| GenBank Accession | AL753849 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:20588803 - go to primer design |
| BLAST e Value | 1e-139 |
| Hit Clone Code (BAC ID) | MBA10 |
| Hit Gene Code | At5g50590 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hydroxysteroid dehydrogenase 4 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | AL753849 [GenBank] |
Gene hit At3g29725
| Sequence (A. th genome BLAST matches underlined) | >39-K012429-022-052-G05-8409 TGATCCATGTAGACTTTCCCGGACATGAAGCACTTTACACTTGAATATATCCTGAAATCT AAAATATTGACTGAGATAGAAGGCTAAGGAGACATTTTGTTTTGAAACAAATACCTGAAA TTGAGTCCAAGTGAAATTGTCTCCATCAACGATGGCTCTTAACATGATCAAGATCTCCCG AGTCAGCCATGCAACTAACTCAGTCACCCCGCTCTTCCTCCCACTCACTTTCTTCGATTT ACTTTGGCTAAAACACCATCCCATCGAGCGAGTCACCTTTTACAAACTCTCTGAGTCATC ATCTCACGACTCTTTCTTCTCCACAATCCTCCCAAAGCTCGAGCAATCTCTCTCCCTTGC CCTCACTAACTTCCTCCCACTCTCTGGTCACATCAAATGGGATCCACAAGATCCTAAGCC ACATGTCATCATCTTCCCACAAGACACTGTCTCTTCACTGTGGTTGAGTCCGACGCTGAC TTCTCAAGACTCTCCGGCAAAGAGCCTCGTCTCGAGAGCAAGGTACGTTCTCTTATCCCT ACGTTAGAGACTTCCTCTAACTCTTCATCTATTCTTGCTCTACAAAATACTCTCTTCCCA AACCAGGGCTTTTGTCTTGGCCTCACACCCATCATGGTCATCTGGACCGGAAAACCTCAA CAAATTTCACAAATCTTGGGCTCATATATGTAAATCCGGACCCCTCCCCAGGGTTTGGTT TTCCTACCCATTCGGTCGGACGGGGATCAATGTTTCTGGCGGACTCAAATTAAAATCTTG CACTCCTGGCGTACTTTCAGGAGAC |
| GenBank Accession | AL753848 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:11556065 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | MOD1 |
| Hit Gene Code | At3g29725 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | 5' region |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At5g22910
| Sequence (A. th genome BLAST matches underlined) | >39-K012359-022-052-G05-8409 TGATCCATGTAGATTTCCCGGACTGAAGGGGATACAACCGATAAGTGATGCCTGATGAGC TGAAGATGTCAAACTCCGAGCTCGGCCGCATCGCCATATCTACGGCCGCCGTCAGCGATT TCCTTGGATTTCTCACTCTATGGGATCCTCCCTATAGTGAGGCGTATTACTCAGTACNGT ANCACTTATGTAGCGGGGAAGAAATAGAATGTGACGTAGGATTGTGAACTTGGTGAGTGC GTTATGAAGAGGTCTATAGTTTGTTGCGGGAAGGAGTAAAATGATGACACCAGAGGAACA GGAAGAGTTGTTCTGGTGGGATTGATCATGAGAGAGAGGAAGAAAGATACATGAGTGGCC GGTGATATCAACGAGAGAAAAGGTGCAGTGTCAGATACATGGGCTTTGTGCATAGAGGAG GTTGACGATTCTATGGGATCCGTTGATAAAGTGAATCTGCCCGCGTGAGTATACTGAGAC TGTATCTTTGATATTTTTGGAGTAGACAAGCGTGTCGTGCTCCACCGAGTTGACGAAGAT TGTCCTTCTTGGTATTGGGCCGTGGGAGACTCTGTATGAACGGGAGTGAAGCAATATACG GGAACATCGTGATAGGGCGTCCTATTGTGAATCTGCGATTGCTGGGGAATCGGGGAGCGG TTTTTGGGAGCTCCCCTGTATGGGCGTCTGAGTTGCTGGGCGTTCCCCCGCCCTCACGGA GACCGAAGCGGTCCAGGGGTCGCCGCGGGGGCCGTCTGC |
| GenBank Accession | AL753847 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:7663114 - go to primer design |
| BLAST e Value | 9e-39 |
| Hit Clone Code (BAC ID) | MRN17 |
| Hit Gene Code | At5g22910 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | cation/H exchanger 9 |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 052G08 |
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Last Updated on 10.06.2021 13:37 |
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