SimpleSearch - Line and FST details
Line specific information
| Line ID | 053A09 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N405001) |
| Segregation Analysis | 100:98:77 |
| Confirmed for Hit | At3g52526 At3g04940 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 97189 |
Gene hit F12F1
| Sequence (A. th genome BLAST matches underlined) | >65-K012472-022-053-A09-8409 NTGATCCATGGTAGATTTCCCGGACATGAAGCACTTTACACTTGAATATATCCTGACAAT GATCCGCATGATTGCATAGTCAGCGATAGCGCATAACACTAATTCTCATTGGCTATGGGA TCCTCCCTATAGTGACTNAAGGAAGGGACGGGCCAAGGAAAGGGTGATGGGACGGCACCC CTTACGTTACGGGAGAGAACACATCACATCACTCGACTTTGATTACAAGACGGGAACGGA ACGTTTTTCCACTACGCTACGCTCGGGTGGGGGGGCATCTTTGGGACCACTGTCGGGAGA GGCATCGTCAACGATGGCCTTTCCTTTATTGCAATGATGGCATTGGCAGGAACCACCTTC CTTTTCCACTATATTAACAATACAGTGACAGATAGCTGGGCAATGCAATCCGACGAGGGG TCCGGATCTTACCCT |
| GenBank Accession | FR801609 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:4094515 - go to primer design |
| BLAST e Value | 0.097 |
| Hit Clone Code (BAC ID) | F12F1 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | FR801608 [GenBank] FR801609 [GenBank] |
Gene hit At3g52526
| Sequence (A. th genome BLAST matches underlined) | >65-K012481-022-053-A09-8409 TGATCCATGGTAGATTTCCCGGACATGAAGCACTTTACAATTGAATATATCCTGCAACCA GGAAAGTGGGAATCCAAACTCCCCATGATAGCAGATAACCAGGTTAGGCCAAGCTCTCCG CCTATGGGATCCTCCCTATAGTGANNNNNNNNNNNNN |
| GenBank Accession | FR801610 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:19485120 - go to primer design |
| BLAST e Value | 1e-22 |
| Hit Clone Code (BAC ID) | F22O6 |
| Hit Gene Code | At3g52526 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | phosphoglucan phosphatase |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
Gene hit At3g04940
| Sequence (A. th genome BLAST matches underlined) | >65-K012485-022-053-A09-35St TGACGTAAGGGATGACGCACAATCCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAA GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG GCTTAAACTATCATTGCACATCTATATTCTGCCATATTTTCGTGAACTTTAAGATTTCAG ATGATGTATGATCTCTAATCTCTATGCGATCCTGCCTACAGTGCTTTGCCATTCCATTGT CGGTTCAATTCTATAGAGTACCTGCCCGCGTCTATAAAATGAAAGGACATTACGATTAGA CGACACATCACGATCCGACCCGTCTATATGGCTAAGGCAACGCACTAAACATACTATTCC GGAAAGCATTATTTCTACGCGACTACGTTACCTTCCCGTTGGGGGCGGGGCTGAGATGCT ACACTATACGAGCCTTGCCTTGACCATTCCCTTACCCCATCCCATCTCCTCATCGGACTG AGCTGATGCTATGCTATACACTCCTCTCATATACTTCTGTCCGTGCAGGCTGCCCTACCA GTGTAGCCCATCACCTTACCTAACACACTCCACTGCTATGCTATGCTACTTTTCCTGGAA AGGGGANNGNGCAGGAACCAGAACCGCCCGCCGCTGGCCCTGCTGGCCTATGCCCCCCGT CAACCCCGACCACCCAGGACTTGGCCCACCCCACGGGGCCGAACTGCCCGCCGGCCGGCT GGCCCCAAGCTGTTTTCCCGAGAAAATACCCGGGCCCCAGGCCCCGACCGACCGGGAACT GGCCCCGGATGCCTGGACCACCCTCCCGCCCTGGCCAACGTTGCGGACCGTGACC |
| GenBank Accession | FR801611 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:1367581 - go to primer design |
| BLAST e Value | 5e-16 |
| Hit Clone Code (BAC ID) | T9J14 |
| Hit Gene Code | At3g04940 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | cysteine synthase D1 |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR801611 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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