SimpleSearch - Line and FST details
Line specific information
| Line ID | 064B02 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N406062) |
| Segregation Analysis | 50:47:34 |
| Confirmed for Hit | At3g63070 |
| Parent of DUPLO pair | 12250 |
| Parent of pair(s) | none |
| Note |
Gene hit At5g35926
| Sequence (A. th genome BLAST matches underlined) | >10-K025917-022-064-B02-8409 TAAAGCTATATGAATCGAAACTCAGATCCCTGATATTTTTCATGTCCCATGTTATGGATA ATCTTGCAAATTTCATTTTTCTATTGTATATAATATTTTTTGATGGGGAATGTATTTAAC ATTTAAATAAAATACTGTTTTGGCTATAATTGTTAAGTTTTTTAAATATGTATTAAGCCA CAAAAAAAATATATACCCCTATTGTAATTTTCTTATTTTTTCAAAAGCCAAACAAAATAT CTTCAAATAGGGTCATCCACCCATAAATAACTACTCTTCCATAACTAACACAAAAACCTT ATATCATAAAAAATATC |
| GenBank Accession | CR396089 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:14062572 - go to primer design |
| BLAST e Value | 2e-51 |
| Hit Clone Code (BAC ID) | F14A1 |
| Hit Gene Code | At5g35926 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Protein with RNI-like/FBD-like domain |
| Insertion Classification | 5' region |
| Confirmation Status | unknown |
Gene hit F15A18
| Sequence (A. th genome BLAST matches underlined) | >10-K026596-022-064-B02-8409 AGACCTAGTGATAACGAATACGCACATACNCACTTGAGTAATCTGCACTATCCAGTCACT ATGGGATCCTCCCTATAGTGAGACTATGGGATCCTCCCTATAGTGAGTCGTATTACTCCC ATGGGCTACACTGAATTGGTAGCTCAAGGTAATGCTTAAGTCATACCTTAACTTTCATTT TATAATAACGCTGCGGACATGTATGGAACCCTGCATATATGGGATGTTTTTGATAGGTTG TGTGTAACGGTCACGTGAAGTACAACATCAAAGGTGCTATATGGGACGCGAATGTTATTA GAAAGATAGCAGGTTCTGGTAGAAGAAACAATAGTAAGATGTAGTGTGAACACGTAGTAA TGATGGTCTTTTATATAAGTCATTTAAATAAAGCGGAGAGAGGGTAATGTAAAGTAGGTG TTTTTTGTGATTTAAAGAG |
| GenBank Accession | CR396090 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:9722018 - go to primer design |
| BLAST e Value | 2e-17 |
| Hit Clone Code (BAC ID) | F15A18 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
Gene hit MSF19
| Sequence (A. th genome BLAST matches underlined) | >10-K025916-022-064-B02-8409 TCGTGTTACCTTCGATGTGTCATATATTAACACAAATGCCGGTGTATGACATCCTCCCTA TAGTGAGAGGTATCCTCCCTATCGTGAGACGACAATCTGATCCCCGGTCTATATTATCCT CCCTACATCGAGTCATTCCCCACGTTCGCTTTGTCATCCTACTATTTTGTTATAATTGCA AAATTGTGAATTATTGTATGACAATACTATAATACACACAACACGTTCAGTTTTGTCTTT TTTTTTTTTACCCTAAAAATTTTTAAAAATAAATACATTTTGCTCCTTATTGAACAATGA AATAGGCTTTTGTTTTTTCCTCCTTCTTCTTTTTAAGGGAACCTTTCTCAACCTTTCAAA TATAACAATATTTTCTTTGCCTAAAAATATTCCAACGGGGACATTCCCTTGTATTTT |
| GenBank Accession | CR396088 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:23260407 - go to primer design |
| BLAST e Value | 3e-62 |
| Hit Clone Code (BAC ID) | MSF19 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit T16L24
| Sequence (A. th genome BLAST matches underlined) | >10-K026597-022-064-B02-8409 GTGATAGATGGAGTCCTATTGATAACGAATACGCTGATACACCCGTGAGTAATCTGCACT ATCCAGTCACTATGGGATCCTCCCTATAGTGAGACTATGGGATCCTCCCTATAGTGAGTC GTATTACTCCCATGGGCTACACTGAATTGGTAGCTCAAGGTAATGCTTAAGTCATACCTT AGCTTTCATTTTATAATAACGCTGCGGACATGTATGGTATCCTCCCTAATGTGATTCTTT TTTGTCCCAAAATTGGTAGGT |
| GenBank Accession | CR396091 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:22046987 - go to primer design |
| BLAST e Value | 2e-11 |
| Hit Clone Code (BAC ID) | T16L24 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At3g63070
| Sequence (A. th genome BLAST matches underlined) | >66-011522-rosso-B2-oriL TTGATCCATGTAGATCTTCCCGGACATTGAAGCATTTACAATTGAATATATCCTGGCACT AATAAAACTGATAGGCCCCATGATCTTTGTCTAATGTTTTGTTTCGCTAGGCCCACACTT TGGAAAGTGAGTCAAATTTACAAAGGAGGGCGGATTTCATCTCTTTCCTCTGAGGATTCT ATTGCTCAGTGCTCCAAAGGTTAGAATGGTGAGATATGCTGTTTTTGCTTAGTAGCATCA TTGATGTTCTCAATGGCGAGTTAACTTTGTGAATACTACCTTTCTATTCAGGTGATGCTG GTGGTGTTTATCTTTCATCCATTCAGGCTATGCTACCTCGCCTATTAACTGCTGCTGTCC CAGCTGGAGCTACCACACAAGAAAACCGAAAACAGTGCTTGAAGGTATGCGATTTTAGTT AGATATTCTTTTTCTTATGAATACTAACTCTCACCCTTTCTGCTCTTATATAGGTTTTGA GGCTTTGGCTTGAAAGACGGATCCTTCCTGAATCTATAGTTCGTCACCATATAAGAGAAC TTGATTCGCTTAGTAATGTTCCTGCTTGCCTCTATTCTCGGCGATCCGCTCGAACAGAAA GGGCGCTGGATGACCCTGTTAGAATATGGANGGGATACTGGTGGATGAATATGGAAGGTG AGAGTTGAGTCTAATCAGGTTTTGGGATCTTGGATTCCTTATCTCTAAGGTCATAAACTT TTTTCACCCTTTTTGGTTCAGAAATTCAACCTCTCCAACTTCATTGGATTCTGCATACCC NCCAATACTTAGGG |
| GenBank Accession | AL755021 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:23307465 - go to primer design |
| BLAST e Value | 0.0 |
| Hit Clone Code (BAC ID) | T20O10 |
| Hit Gene Code | At3g63070 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Tudor/PWWP/MBT domain-containing protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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