SimpleSearch - Line and FST details
Line specific information
| Line ID | 088G03 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N408427) |
| Segregation Analysis | 50:36:34 |
| Confirmed for Hit | F22F7 At1g68440 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | none |
| Note |
Gene hit F22F7
| Sequence (A. th genome BLAST matches underlined) | >23-K016184-022-088-G03-8409 GATCCTGTAGATTTCCCGGACTGAATGCATTTACAATTGTGAATCTTAATCACGTTTGTA GTGATAATCTAATCACGTTTGTTTTTAATGATAATGCGTTGTCCCCATCCTTGTTCTTAA TTCAATTGTCAATCACCTCCATTAATTTTGTGTTCACCTCGGTTAGAAAGCTTTGTTTTA ATTTGTTAAGACGATGCTGATATATCCTGAATCATGATTAAATTAAAATCCTTAAGTTAA AGTCATTACAAAAACCACATTTGAATATTGATCAGATATTGGTCCCATTGCCCAAAACGA TGGTGCAAAATGTCTAAAGAGTGATAAGCTA |
| GenBank Accession | AL937723 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:1601589 - go to primer design |
| BLAST e Value | 9e-153 |
| Hit Clone Code (BAC ID) | F22F7 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At4g36210
| Sequence (A. th genome BLAST matches underlined) | >23-K016185-022-088-G03-8409 TATCCTGAAATTTCCCGGACATGAAGCCATTTACATTGAATATATCCTACACTATACGAT TACCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC TGTCCTCTCCAAATGAAATGAACTTCCTATGGGATCCTCCCTATAGTGAGAGATTAAAAA AAGAGAAATTTCCAATTTTGTATTAAATTCAATAATTAATCCTTCGTTCCTTTATCAATC TGTTGCTGTTTCCGAAATCGAAAATTTACCCCAAATTTATGGTTTTTGAAAATGGGGAGT TTTAATTATCGAATTTCTAGTTAATTTCCAAACTTTGTTAAAACCTCAAAAGCCAAGGGA ATCCTCCCTATAGTGAGAAATGGAATCCGAGGAGGACTTCCGGATATTACCCTTTGCTGA AAAGTATCCATTGCTCCTTTAATATAATGAGACTGTATCTTTGATATTATTGGAGTAGAC AAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGAG ACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAAGTCCTCTATTTGAAT CTTTGACTCCATGGGAATTGGGGATCCTCTAGTGCCTAGTTGGAAGCACACTGATTATCC ATATAGTGCAAAAAGGGAACTCTTCTACTCTTATTCACCTCAAAAAATGCTGCAACCGCA AGGGAACCAACAACAGTT |
| GenBank Accession | FR802687 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:17132717 - go to primer design |
| BLAST e Value | 3e-32 |
| Hit Clone Code (BAC ID) | F23E13 |
| Hit Gene Code | At4g36210 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | transmembrane/coiled-coil protein (DUF726) |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g68440
| Sequence (A. th genome BLAST matches underlined) | >23-K016185-022-088-G03-8409 TATCCTGAAATTTCCCGGACATGAAGCCATTTACATTGAATATATCCTACACTATACGAT TACCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC TGTCCTCTCCAAATGAAATGAACTTCCTATGGGATCCTCCCTATAGTGAGAGATTAAAAA AAGAGAAATTTCCAATTTTGTATTAAATTCAATAATTAATCCTTCGTTCCTTTATCAATC TGTTGCTGTTTCCGAAATCGAAAATTTACCCCAAATTTATGGTTTTTGAAAATGGGGAGT TTTAATTATCGAATTTCTAGTTAATTTCCAAACTTTGTTAAAACCTCAAAAGCCAAGGGA ATCCTCCCTATAGTGAGAAATGGAATCCGAGGAGGACTTCCGGATATTACCCTTTGCTGA AAAGTATCCATTGCTCCTTTAATATAATGAGACTGTATCTTTGATATTATTGGAGTAGAC AAGCGTGTCGTGCTCCACCATGTTGACGAAGATTTTCTTCTTGTCATTGAGTCGTAAGAG ACTCTGTATGAACTGTTCGCCAGTCTTTACGGCGAGTTCTGTTAAGTCCTCTATTTGAAT CTTTGACTCCATGGGAATTGGGGATCCTCTAGTGCCTAGTTGGAAGCACACTGATTATCC ATATAGTGCAAAAAGGGAACTCTTCTACTCTTATTCACCTCAAAAAATGCTGCAACCGCA AGGGAACCAACAACAGTT |
| GenBank Accession | FR802687 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:25658016 - go to primer design |
| BLAST e Value | 6e-18 |
| Hit Clone Code (BAC ID) | T2E12 |
| Hit Gene Code | At1g68440 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | transmembrane protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
|
Last Updated on 10.06.2021 13:37 |
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