SimpleSearch - Line and FST details


Line specific information

 
Line ID 114C06
Vector Used pAC161
Line Availability available as T3 set from NASC (N410878)
Segregation Analysis 50:47:44
Confirmed for Hit At5g23020
Parent of DUPLO pair none
Parent of pair(s) 9525

Gene hit MPE11

 
Sequence (A. th genome BLAST matches underlined) 
>43-K012504-022-114-C06-8409
NTGATCCATGTAGATTTCCCGGACATGACATGCCTGTAGCACTTGATAGTACCTCAGCAC
TGAAACACAGATCACTTTATATCACTGCGTCAGAATGTGGTAAAATAAGTCCGATTGCAC
CCGATGAATATGGACTAGCGTATTATAGGGTATACCTTTGCATTGTTTGGGCTGCGCACA
CCATGAACAAACATTTGGTATAACGCTAGCGAACACTAATATACTAAAAATATCTAACAT
TTTTTGCTCGAAACTATTTGGCGTCGGACCTAATAGTTCCGAATCAACTGAACGGGGTCG
CTTCCATATTAATCCAAAAAGTAAACATCTATATGGGATCCTCCCTATAGTGAGTCGTNN
TTACTC
GenBank Accession FR803318 [GenBank]
Graphic View Graphic view of sequence FR803318 of line 114C06
Predicted Position of Insertion Chr3:9473881 - go to primer design
BLAST e Value 4e-13
Hit Clone Code (BAC ID) MPE11
Insertion Classification Genome Hit
Confirmation Status unknown

Gene hit At5g23020

 
Sequence (A. th genome BLAST matches underlined) 
>43-K012566-022-114-C06-35St
TGACGTAAGGGATGACGCACAATCCACTATCCTTCGAAGACCCTTCCTCTATATAAGGAA
GTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCG
GTTTAAACTATCAGTGTTTGATACGACGGATCGTAATTTGTCGTTTTATCAAAATGTACT
TTCATTTTATAATAACGCTGCGGACATCTACATTTTTGAATTGAAAAAAAATTGGTAATT
ACTCTTTCTTTTTCTCCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCG
GACATGAAGCCATTTACATATCATTGTTTTATATTTATAATCTTGTCACCTCGATAATTT
AAATTTGTTCGGAATTTTTGTTGATTAATCTAACTCAGACAAAGGGAGAGCAATTACGTA
CGGATATTGTGTTGGCGGTAGCAACACCAAGGTCGTTATGACAATGAATGGCGAAGACAA
TATCATCAGCCCCTGGAGTGTTTTCTATGACATAGGCCACGAGTTCTCCGAATTCTTGCG
GCATGTTGATCCCGACCGTGTCCGCAAACCCCACCGTGGTTGCACCCGCTTTTATCGATT
CTTCTATGGGATCCTCCCTTAGTGA
GenBank Accession AL756869 [GenBank]
Graphic View Graphic view of gene At5g23020
Predicted Position of Insertion Chr5:7719923 - go to primer design
BLAST e Value 1e-161
Hit Clone Code (BAC ID) T20O7
Hit Gene Code At5g23020 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation 2-isopropylmalate synthase 2
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37