SimpleSearch - Line and FST details


Line specific information

 
Line ID 142B06
Vector Used pAC161
Line Availability available as T3 set from NASC (N413554)
Segregation Analysis 140:85:63
Confirmed for Hit At3g52720
Parent of DUPLO pair none
Parent of pair(s) none
Note

Gene hit At3g52720

 
Sequence (A. th genome BLAST matches underlined) 
>78-K020160-022-142-B06t-8409
GGGATCCTCCCTATAGTGAGATTTCATACAAAAATGTCAAATAAACATGAAAGTAGTAGC
TAGCTAGATGTTATCAATCGTATATATTGACTTCTCTATTCATTCAAACGTGAATAACAA
GTTTGACATAAAGGGTTTAGCTTAAAAGCAGGGGTTGTGGAGCCCAAAAGACACTCAAAT
TGGTTTGAATATCGTCCAGAAAAATTAATAATGTATTAAGTTTGAAACTTCAATATCGTT
ACGTACACTATGGGATCCTCCCTATAAAGGGACTATGGGATCCTCCCTATAGTGAGANNN
TGTTTTTATTC
GenBank Accession BX530791 [GenBank]
Graphic View Graphic view of gene At3g52720
Predicted Position of Insertion Chr3:19539036 - go to primer design
BLAST e Value 6e-127
Hit Clone Code (BAC ID) F3C22
Hit Gene Code At3g52720 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation alpha carbonic anhydrase 1
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details
Other FSTs Supporting this Hit BX649718 [GenBank]

Gene hit At4g17140

 
Sequence (A. th genome BLAST matches underlined) 
>42-K012895-022-142-B06-35St
TGACGTAAGGGATGACTGCACAATCCCCTATACCTTCGAAGACCCTTCCTCTATATAAGG
AAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTT
CGGTTTAAACTATCAGTGTTTGACAGGAGATATTTGTTCATGAATATGTGACTGACACAA
GCGTTTCTGGTCATATGCCTCAATTGTCTATGGGATCCTCCCT
GenBank Accession AL765669 [GenBank]
Graphic View Graphic view of gene At4g17140
Predicted Position of Insertion Chr4:9634945 - go to primer design
BLAST e Value 4e-13
Hit Clone Code (BAC ID) FCAALL
Hit Gene Code At4g17140 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation pleckstrin homology (PH) domain-containing protein
Insertion Classification CDSi
Confirmation Status failed


Last Updated on 10.06.2021 13:37