SimpleSearch - Line and FST details


Line specific information

 
Line ID 221F09
Vector Used pAC161
Line Availability available as T3 set from NASC (N421189)
Segregation Analysis 50:29:22
Confirmed for Hit At2g47800
Parent of DUPLO pair none
Parent of pair(s) 2658, 84554

Gene hit At2g47800

 
Sequence (A. th genome BLAST matches underlined) 
>70-K014204-022-221-F09-8409
TTGCCTATGGGAGGCTGACGTTGCTGAGCACGGTTCCTTCAAAGAGGACAGGTTCTTGCG
GAATGATTCCGAATCTTGACCTGAGATCATGTAGCCCTATGGGATCCTCC
GenBank Accession AL767747 [GenBank]
Graphic View Graphic view of gene At2g47800
Predicted Position of Insertion Chr2:19579825 - go to primer design
BLAST e Value 3e-32
Hit Clone Code (BAC ID) F17A22
Hit Gene Code At2g47800 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation multidrug resistance-associated protein 4
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details

Gene hit MCM23

 
Sequence (A. th genome BLAST matches underlined) 
>70-K014206-022-221-F09-8409
GATTGGAACAGTGGATGATAACTGGGGATAACTGGATCGTAAGGCACAGGCGGCTTCTAT
GCTTAAGCAGGCGCCTGCCGAAACGCAACATACTACTCTTGCCTATGTCCTTGTGAAGAT
GGAACCTACAGCACCCCTAAATATGAAACGTTCCTGCGCACTGATCGGCTATCTATAGCC
AGACATGTTGCAAAAAGTTGGGTTCCAGAGATCTCACTACACAACTAAATGACTCCAAAA
GCCAGCTATGGGATCCTCCCTACTGATGAGACCAATAAAGTGACAGATAGCTGGGCAATG
GAATCCGAGGAGGTTTCCGGATATTACCCTTTGTTGAAAAGTCTCAATTGCCCTTCGGTC
TTCTGAGACTGGATCTTTGATATTTTTGGAGTATACACGCGTGTCATGCTCCACCATGTA
GACGAAGATTCTCTTCTTGTCATTGAGGCGGAAGAGACTCTGTATGAACTGTTCGCCAGT
CTATACGGTGAGTGCTGCCAGGGTCTCTATTGGAATCTTTGACTCCATGGGAATTGGGGA
TCCTCTA
GenBank Accession AL767748 [GenBank]
Graphic View Graphic view of sequence AL767748 of line 221F09
Predicted Position of Insertion Chr5:5976891 - go to primer design
BLAST e Value 1e-10
Hit Clone Code (BAC ID) MCM23
Insertion Classification Genome Hit
Confirmation Status unknown


Last Updated on 10.06.2021 13:37