SimpleSearch - Line and FST details


Line specific information

 
Line ID 270A06
Vector Used pAC161
Line Availability available as T3 set from NASC (N425830)
Segregation Analysis 78:61:49
Confirmed for Hit At4g30935
Parent of DUPLO pair none
Parent of pair(s) 96990, 96997

Gene hit At1g25220

 
Sequence (A. th genome BLAST matches underlined) 
>41-K015070-022-270-A06-8409
CTGATCCATTGTAGATTTCCCGGACATGAANGCCTTTACAATTGAATATATCCTGGACAA
ATCTACTTGCATCAATGATTCATTTGGAGGTTAGTGGTTTATAAACTATGACATCTTATT
TCTCTCGTATCATGGATCTATAACATGGATTTGCTCTTTATTATGTATCCACAACTTAAA
TGCAATTCTGTTTGAACATTGATTACGAACTATTGTGCGGTCACCACTTTGGTGTCATGC
ATGGGAAAAGCTCACTGGGTCACTATGATGAGACAGGATGATAGCGACTTGTACTCTGGA
TTATCAAAGTATGACTCACAGTTTTTTCTGTGTAGTCTAATCTACCATTGCATAGGTCTG
TAAAAATGTGTAATAAAACAAATCTTGTTGGTTTCAGCCCTTTCATTGTAGGTAGATATC
ACAGTCTCGTGATCGAAAAAGATACATTTCCTATGGGATCCTCC
GenBank Accession AL942899 [GenBank]
Graphic View Graphic view of gene At1g25220
Predicted Position of Insertion Chr1:8838092 - go to primer design
BLAST e Value 6e-117
Hit Clone Code (BAC ID) F4F7
Hit Gene Code At1g25220 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation anthranilate synthase beta subunit 1
Insertion Classification CDSi
Confirmation Status unknown

Gene hit At4g30935

 
Sequence (A. th genome BLAST matches underlined) 
>41-K015063-022-270-A06-8409
GATCCTGTAGATTTCCCGGACATGAAGCCATTACACTTGGATAATCTAAGACGACAAAAG
GGTGTATTTGAATAATCGCGATGATTTAGAAGGGCGTGAAAAGCGTCTGTCCGTTACTCT
CTCATTTGTATGTGCATGCCAACCACAGGGTTGCCCCTCAGTAGCGTTTGGCATTCCGAG
CAATAATGACTTCTGTGCAACCATCCTCACAACGTCGGACAGCCTGACGACTGAGCATCA
TTCCCAAAATATCCCTTGGATCGTCTGGTCCGGCTATGGAGATGCTCCCTATTCAGAGAT
GTATTCTTTAGTAGAAGCAGACGGATCTGAACCGCTATGGGATCCTCCCNTATAGTGAGA
GenBank Accession BX285303 [GenBank]
Graphic View Graphic view of gene At4g30935
Predicted Position of Insertion Chr4:15052679 - go to primer design
BLAST e Value 3e-14
Hit Clone Code (BAC ID) F6I18
Hit Gene Code At4g30935 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation WRKY DNA-binding protein 32
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37