SimpleSearch - Line and FST details


Line specific information

 
Line ID 314H04
Vector Used pAC161
Line Availability available as T3 set from NASC (N430136)
Segregation Analysis 50:22:8
Confirmed for Hit At5g42260
Parent of DUPLO pair none
Parent of pair(s) 69965, 70004, 70038, 70071, 70166, 70275, 70328, 70547, 70579, 70625, 70643, 70658

Gene hit At2g01008

 
Sequence (A. th genome BLAST matches underlined) 
>32-K015823-022-314-H04-8409
TGATCATGAGATTTCCCGGACATGAAGCCATTTTTGTAGCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTATGGGATCCTCCCTATAGTGAGANGTTAATCTATAAATAGAGAAATTA
TAAGATAGATATAGTATAACAACAAATCTTTTAAGTTTTACTTGTTCATAATTAGTAAAC
AGAACATTCAAATGATATTATCTAGGAACTGGAACGAGACGGGTGGCAAAGATTTCTAGT
ACCACTTCATACTACCGTGGGTTTTTTAAACCTTCCGAGTTTTGTTGATGTTATTCCGAG
AATTAGCAAACCGTAACAAAGATGTTCTTGGCAACCATCTTTTGATGGGAGTCCGGCTGT
TCGATAGCCGGCCAAGGGTGATGAACGAAATGTGAACCCTTGTCTCGCCTATGGGATCCT
CCCTATAGTGAG
GenBank Accession AL948734 [GenBank]
Graphic View Graphic view of gene At2g01008
Predicted Position of Insertion Chr2:2567 - go to primer design
BLAST e Value 8e-101
Hit Clone Code (BAC ID) F23H14
Hit Gene Code At2g01008 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation maternal effect embryo arrest protein
Insertion Classification TS2TE (3')
Confirmation Status unknown

Gene hit At5g42260

 
Sequence (A. th genome BLAST matches underlined) 
>32-K015819-022-314-H04-8409
TTATAGCATAGAAAGATATATAGTTCTCATTCTTAATTTTCTTTTAATTGACTGTTGAAA
CTTGTTTTGTTGTAAAAAGATGAATAAAGCTCAGATTTATATAGGCAAGAGAAATGTTAT
CGCAATTTGTTGTGTTAATCTATAAATAGAGAAATTATAAGATAGATATAGTATAACAAC
AAATCTTTTAAGTTTTACTTGTTCATAATTAGTAAACAGAACATTCAAATGATATTATCT
ATGGGATCCTCCCTATAGTGAG
GenBank Accession AL948733 [GenBank]
Graphic View Graphic view of gene At5g42260
Predicted Position of Insertion Chr5:16898738 - go to primer design
BLAST e Value 2e-129
Hit Clone Code (BAC ID) K5J14
Hit Gene Code At5g42260 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation beta glucosidase 12
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37