SimpleSearch - Line and FST details


Line specific information

 
Line ID 321D12
Vector Used pAC161
Line Availability available as T3 set from NASC (N430768)
Segregation Analysis 50:48:47
Confirmed for Hit At2g20750
Parent of DUPLO pair none
Parent of pair(s) 7975

Gene hit At3g41768

 
Sequence (A. th genome BLAST matches underlined) 
>92-K015945-022-321-D12-8409
TATCATGAAATTTCCCGGACATGAAGCCATTTACATTGAATATATCCTGAGGGAGACTGG
GCGGATGGTAATAATGGGGAGTGCTCTCTGAAAGCGAGATAGGCCTTGTCCAGACATCCT
CGATCTTGAATCATGCACCGCAGCCTTCACCACCTTTGAACAGAAGGGGAGTCACTGTTC
CAACACTATGGGATCCTCGGCGATAAAGAGAGAATGATGCGTCGCCAGCACAGAGGCCAT
GCGATCCGTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTT
ATCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACGTATTAGCTCTATGGGA
TCCTCCTTATAGTGAGACCACCANNCTGCGGTGTGCCTCGTAAATATTAGGATATAGCGA
TGGGTGCACGAAAACCATGTATATCGCTCAAGTAGCCCACGCCGCTACTTGATCGCATAT
CACTGGGTTTCCGGTTGGGAGCTGTCGATTGAAACACGGTGCATCTGATCGGACAGGGCG
TCTAAAAGCGGCGCAATACGGCTGATCTCATCGGCCCGGCGATACATGGCTTCGCGTCCG
GGATGGCTGGCGGCCGGTCCGACATCCACCACCTCTGATCCGACTCGCACGATTTCCATC
NGCCGGGGTGACAGCGCCCGGCGGGGTCTA
GenBank Accession AL949528 [GenBank]
Graphic View Graphic view of gene At3g41768
Predicted Position of Insertion Chr3:14198131 - go to primer design
BLAST e Value 9e-76
Hit Clone Code (BAC ID) F21A14
Hit Gene Code At3g41768 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation rRNA
Insertion Classification TS2TE
Confirmation Status unknown
Other FSTs Supporting this Hit AL949529 [GenBank]

Gene hit At2g20750

 
Sequence (A. th genome BLAST matches underlined) 
>92-K015945-022-321-D12-8409
TATCATGAAATTTCCCGGACATGAAGCCATTTACATTGAATATATCCTGAGGGAGACTGG
GCGGATGGTAATAATGGGGAGTGCTCTCTGAAAGCGAGATAGGCCTTGTCCAGACATCCT
CGATCTTGAATCATGCACCGCAGCCTTCACCACCTTTGAACAGAAGGGGAGTCACTGTTC
CAACACTATGGGATCCTCGGCGATAAAGAGAGAATGATGCGTCGCCAGCACAGAGGCCAT
GCGATCCGTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTT
ATCTAATAAATGCGTCCCTTCCATAAGTCGGGGTTTGTTGCACGTATTAGCTCTATGGGA
TCCTCCTTATAGTGAGACCACCANNCTGCGGTGTGCCTCGTAAATATTAGGATATAGCGA
TGGGTGCACGAAAACCATGTATATCGCTCAAGTAGCCCACGCCGCTACTTGATCGCATAT
CACTGGGTTTCCGGTTGGGAGCTGTCGATTGAAACACGGTGCATCTGATCGGACAGGGCG
TCTAAAAGCGGCGCAATACGGCTGATCTCATCGGCCCGGCGATACATGGCTTCGCGTCCG
GGATGGCTGGCGGCCGGTCCGACATCCACCACCTCTGATCCGACTCGCACGATTTCCATC
NGCCGGGGTGACAGCGCCCGGCGGGGTCTA
GenBank Accession AL949528 [GenBank]
Graphic View Graphic view of gene At2g20750
Predicted Position of Insertion Chr2:8941649 - go to primer design
BLAST e Value 4e-10
Hit Clone Code (BAC ID) F5H14
Hit Gene Code At2g20750 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation expansin B1
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37