SimpleSearch - Line and FST details
Line specific information
| Line ID | 338G05 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N432429) |
| Segregation Analysis | 50:35:30 |
| Confirmed for Hit | At3g08840 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 7965 |
| Note |
Gene hit At5g53370
| Sequence (A. th genome BLAST matches underlined) | >39-K016159-022-338-G05-8409 GGACTGAAGCCTTTACATTGAAACNACAAACGTGAAGAGAGAAAAAAAAAAAGTGAAATA ATTGAGGAGGGATCTCTAGGGGATCCTCCCTATAGTGAGATATGGGGGACCATTTTGCCC CCCAAGGTTGGTTGGAGGGAAATGGCCCGTTTCCTTTGAACTCTTTGAACTATGGGGAGT TTTTGAACAAGGGGTTGGGTTCAGGAATTGGCCACCAAGCCAATGGGCCTGGTTTTCTTG TTTTTCCCCCAACGGAAAAGGTTTTGGGATCCTCCCTTATAGTGAGAATTCAACTGGNGG TCTGGGTTATGATTGATTTTAAAACTTTAGGCCCCGCCCCATTCTTGAAGCCCTAAGTAC CGTTCCCTTGTAAAAAAACCCAAGCCCAAAAACTCTTTTGGCTTTTGCCTCCCCCTTGAA ACCTTTGAAAACCAATAAAAAGGATTGTTAGGGTTTTGGAGCCAATTATAAGTTGATTCC ATGGGTATGATGGAGTAAAAAAACTTAAACCGCACCCCAATCTTAAAACCCTAAGAAGGG TTTCCTTGTATAAAACCCCAAGCCAAAAACTCTTACGGACTTTGCCCACCCCTTAAAAGC TTTTTAAACTTTTGGAATCCCCCTTATAAGGGAGACNCNCCATTNCCTGCCCCTCCCCGC CCCCCTTTTTTCTTTATCAAAT |
| GenBank Accession | AL951810 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr5:21649990 - go to primer design |
| BLAST e Value | 2e-14 |
| Hit Clone Code (BAC ID) | K19E1 |
| Hit Gene Code | At5g53370 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | pectin methylesterase PCR fragment F |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At3g08840
| Sequence (A. th genome BLAST matches underlined) | >39-K016137-022-338-G05-8409 TTTCCCGGACTGAAGCCTTTACATTGAAACNACAAACGTGAAGAGAGAAAAAAAAAAAGT GAAATAATTGAGGAGGGATCTCTAGTTTATTTTCCTATTTATTTATGGGGGAATTTTTTT GTCCTCGAAACTGGGATAAAATTTTTTTGTTTTGGTTTCGGCACTAAAAAGCATCCCCAA AAGGGAAAAACAATCCTTAACGGAATAACCTAATGGATCCCCTTGCCGGGCGAAATAAAA GGGCCCTTACCCCCCCATTAGTCCCCCCTTAAAGTTGCTCACCATTGCCTATATAGAACT TTTGGGCAAACTCTTTTATGGATTTCATAGGCCATTTCCGGGGTGAAAAATTGAAACCCT TGTTTAGGGAAAAAC |
| GenBank Accession | AL951809 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr3:2684645 - go to primer design |
| BLAST e Value | 0.001 |
| Hit Clone Code (BAC ID) | T16O11 |
| Hit Gene Code | At3g08840 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | D-alanine-D-alanine ligase family |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit T6D22
| Sequence (A. th genome BLAST matches underlined) | >39-K016137-022-338-G05-8409 TTTCCCGGACTGAAGCCTTTACATTGAAACNACAAACGTGAAGAGAGAAAAAAAAAAAGT GAAATAATTGAGGAGGGATCTCTAGTTTATTTTCCTATTTATTTATGGGGGAATTTTTTT GTCCTCGAAACTGGGATAAAATTTTTTTGTTTTGGTTTCGGCACTAAAAAGCATCCCCAA AAGGGAAAAACAATCCTTAACGGAATAACCTAATGGATCCCCTTGCCGGGCGAAATAAAA GGGCCCTTACCCCCCCATTAGTCCCCCCTTAAAGTTGCTCACCATTGCCTATATAGAACT TTTGGGCAAACTCTTTTATGGATTTCATAGGCCATTTCCGGGGTGAAAAATTGAAACCCT TGTTTAGGGAAAAAC |
| GenBank Accession | AL951809 [GenBank] |
| Graphic View | 
|
| Predicted Position of Insertion | Chr1:2523583 - go to primer design |
| BLAST e Value | 4e-07 |
| Hit Clone Code (BAC ID) | T6D22 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
|
Last Updated on 10.06.2021 13:37 |
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