SimpleSearch - Line and FST details
Line specific information
| Line ID | 347E11 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N433275) |
| Segregation Analysis | 50:35:26 |
| Confirmed for Hit | MLM24 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 5316, 5329, 5386, 8646, 10356, 11341, 95807, 95813, 95842, 95851, 95852, 95853, 95854, 95855, 95856 |
| Note | |
Gene hit At2g25450
| Sequence (A. th genome BLAST matches underlined) | >85-K016241-022-347-E11-8409 TGTCCTGAAATTTCCCGGACATGAAGCCTTTCACATTGAATTTGTTGGAGTTGTTGTTAA CTACTATAAAAACTCGTTTTAAAAATTCTATAATAACTATGGGATCCTCCCTATAGTGAG AAATAAATTAACCCATAGTATTCTTACTCCCAAGCTCCCGTGAGCCCGGTGCTTTTGGAA GCTCCTAGACCTCGAAACCACTTACCCGTATCTCTGCCAACTCGCATCGAGAGACTCATA GAGATCGGCTTTATAGTGATATCGAACCCTTTTGGATTTCTCTCGGGAAGAGACCATACT CCTCACTTCGGGAGCTTGGTCTTGAAACCGTCTGATGCCATATTATCATCTTCTCCATCA CATCATAGCGATACCCCATGGATAATCACCTGGAAAAGTCCACACGCCTCCATTGCGTCT TGAAGCTGCGCAACTACAGGTCTCTCCCACGACCGAGGAT |
| GenBank Accession | AL952985 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:10830972 - go to primer design |
| BLAST e Value | 1e-28 |
| Hit Clone Code (BAC ID) | F13B15 |
| Hit Gene Code | At2g25450 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
Gene hit At1g52630
| Sequence (A. th genome BLAST matches underlined) | >85-K016235-022-347-E11-8409 TGATCCTGAAATTTCCCGGACATGAAGCCATTTACATTGAATATATTCGGAGTTGTTGTA ACTACTATGAAAACTCGATATAAACAATTGCTATAATAACTATTTCATGCACTCTGATGA GAAGCCTCTTGGTTAACGAGAACAGACTTTGTGAACACATTTGTATAAACGGATGTGAAT CCTTCCATCTCCATTAGACCGTCTCCAGCTGCTAAGTATATGTTTGTGCTTGTTGGGATG ATAAGTGACTGAAGCATCAACACTGCTTCATTAGGAGTCAGAGGGCATTTGCCTCGCTTT CTCCATGTCTGAGCTATGGGATCCTCCCCTATAGTGAGAGGCTGGTTATTCAAACCCAGC AATTGGCTGCCGGATTTCGTCCTGCAATGAAGCAGATCGCTTCGGGGGGACACTGTGGCG TGGCTGAATGTAGAGCAGGCCGCCCCTACAGCGTTTGATCAGTAGATTATTTAGAACTTC GGAAGATGGTCAAGGTACGAAGTGACAAGCCACTGCGCTATGGGAT |
| GenBank Accession | AL952984 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:19606700 - go to primer design |
| BLAST e Value | 7e-95 |
| Hit Clone Code (BAC ID) | F6D8 |
| Hit Gene Code | At1g52630 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | O-fucosyltransferase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | failed, line belongs to a contamination group, please check line 347E12 |
Gene hit MLM24
| Sequence (A. th genome BLAST matches underlined) | >85-K016241-022-347-E11-8409 TGTCCTGAAATTTCCCGGACATGAAGCCTTTCACATTGAATTTGTTGGAGTTGTTGTTAA CTACTATAAAAACTCGTTTTAAAAATTCTATAATAACTATGGGATCCTCCCTATAGTGAG AAATAAATTAACCCATAGTATTCTTACTCCCAAGCTCCCGTGAGCCCGGTGCTTTTGGAA GCTCCTAGACCTCGAAACCACTTACCCGTATCTCTGCCAACTCGCATCGAGAGACTCATA GAGATCGGCTTTATAGTGATATCGAACCCTTTTGGATTTCTCTCGGGAAGAGACCATACT CCTCACTTCGGGAGCTTGGTCTTGAAACCGTCTGATGCCATATTATCATCTTCTCCATCA CATCATAGCGATACCCCATGGATAATCACCTGGAAAAGTCCACACGCCTCCATTGCGTCT TGAAGCTGCGCAACTACAGGTCTCTCCCACGACCGAGGAT |
| GenBank Accession | AL952985 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:8362946 - go to primer design |
| BLAST e Value | 3e-26 |
| Hit Clone Code (BAC ID) | MLM24 |
| Insertion Classification | Genome Hit |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
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Last Updated on 10.06.2021 13:37 |
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