SimpleSearch - Line and FST details
Line specific information
| Line ID | 908D02 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N487110) |
| Segregation Analysis | 50:49:47 |
| Confirmed for Hit | At1g20515 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 4975, 4978, 4996, 5029, 5070, 7454, 7459 |
| Note |
Gene hit F21A14
| Sequence (A. th genome BLAST matches underlined) | >12-K030792-022-908-D02-8409 GCGGGTACGGGATCCTCCCTATAGTGAGTCGTATTACTCCCTCCAATGTTGTTTCCCCCT GTTCACCTACGGAGACCTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTTAGTGGA CTTCTCGCGACGTCGCGGGCGGCGAACCACCCACGTGGGCGCGATCCGAACACTTCACCG GATCATTCAATCGGTAGGAGCGACGGGCGGTGTGTATGGGATCCTCCCTATAGTGAGACT TATTTTTCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT ATTTTTTTTATTTTATTTTGTTTTTTTTTNTTTTTCTTT |
| GenBank Accession | CT026404 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:14199558 - go to primer design |
| BLAST e Value | 6e-76 |
| Hit Clone Code (BAC ID) | F21A14 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
| Other FSTs Supporting this Hit | CU457933 [GenBank] CU457931 [GenBank] |
Gene hit At1g20515
| Sequence (A. th genome BLAST matches underlined) | >12-K101542-0022-908-D02-8409 TGAGCCATTACATTGAAATATCCTGACTGAAATGTGAAGCCGGAAGGTAGGGTTCCGGTG AACCTCTCTCTCTTTGTCTCTCTGTAATTGAGCATGTTAGGGATATGTTTCTATGGGATC CTCCCTATAGTGAGACGAAGNACTCACCGCCGCAAGAGAACAACGCGGAAAAAACAAAAA ACAACCGCACTCTAGACCAAAACGCAGAGAAACAAAAAAAAAAAAACGATCAACCAACGA AAAAGACAGAAAAAAAAACAAAGAACCAAACAGAAAAACAATACAAAAACACAACCAGCA ACGAATAACGCCAAAAGAAGGCGAAAGAAAAACAAAACAACGCAGACATAAAAGAAAAAA CGAAAGAACAAACCAACAACAAAAAACCTAAAACAGAAAAAAACAAGAAAAAATAACGAA CAAAAAAAAACAAAAAAACAAAACAAAAAGACAAAAAAAACACGAAAGACGAAACAGCGT ACAGAAGCAAAAGACAAAAAATGACATATAAATACAAAACAAGAACAAAGGAAGAGAAAA CACAAAAAAAACCAAAAAAAAGACAAAAAAAACAAACGAGAAAGAAATTAAAAAACAAAA TAAACAGAAAGAAGCAAAAAACAAAACATAAAAACAAACAGGACACAGAGCAGAAAAACG TAACAAAGAAACAACAAACACCACGAACAAAGTAAAAAAACAAGAAAAAAACAAAAAACA AAAAAACAGCAAAAAAAAAGAACAATAAAAGAATCACAAACAAAAACAAAACAGTAAACA ACAACACAAAGAGAACAGACACAAACAAAAAAAACACAAACAATGAAAGAACAAAGACAA AAATAAAAAACAAAAACATAAGGAGAAAAGCAAACGAAAAAACAAAAAACAACATAAGGA GAAACAAAAAGACAACACAGACACAAACAAAACAAACGACAAAAAAATAACCAAAACAAA AGACGAAAAAACAGCGAAACAAGAAACAGAAAAAGGAAAAAACAAAAAAAGACAGAAACA ATAAAAATAAAATAACCAGAACAACACAAAAACAGGCAAACAAAAACACAAAAGCAAAAA AGACAGAAGACAGCAAAAGAAAAAACCAACGCAAAACAACATAACTACCACACACCAGAA GATAAAATAACAAAAAAAAAGCCACAGGAGAAAAGAAGAAACAACAACAATCAACACGAC CACAAACAAGAAACATAAAACGAAAGAGAAACACACAAAAAACACAACAACAAAAAACAC TTCGAGGAAAAATACACAAATAACAAGGAAAAACAACGACATAAACAACGACCAAAAAAA CCAATTCGTCAAACAACAAAGGACAACCGGAAGAGCAAAGAACGAAATGACGAACGACAA CACAAATGATCAAACACGAGCAAAGACAACTAGAACACTAATGCAAAACACTCACAACAA ATAAGTCATCAGCACAAATACCAAACGAATCGCAGACGCACGAAACACGCAGATCAGAAC ACATACGAAATACAAACA |
| GenBank Accession | CU457932 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:7106187 - go to primer design |
| BLAST e Value | 1e-14 |
| Hit Clone Code (BAC ID) | F5M15 |
| Hit Gene Code | At1g20515 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Natural antisense transcript overlaps with AT1G20520 |
| Insertion Classification | TS2TE |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At3g12780
| Sequence (A. th genome BLAST matches underlined) | >12-K030969-022-908-D02-8409 NNTTTTCCGATTAATCCGGACATGAGCCATTTACAATTGAATATAAAATATTATTATATT TCTTTTTTGAAAAACTTTCAAAGTATCTCACCAAGGGAGATGGCTTAACCTTCCAATGAT TGATAATCAAATTCAAACCCGGCATCACAAATGTTACTATTGACAATAAAACATTGTTGC ACTTGAATTGGGTAGGGGACCCCCCCTACAGTGAGCCCAATTACCCCCTTTTCATTCCTT AAATCCATCGGCACTGTTGCTTCCTCCGCCGGAACTCGCGCACGTGCCTCCCTTCTGCCA ATTCCCTCTACCTCTGTTTCCGCGCGTCCTCTAGGCTTCTCCGCCACTCTAGATTCCCGT CGTTTCTCTCTCCACGTTGCTTCCAAAGTTGAATCGGTGCGTGGGAAGGGAAGCAGAGGA GTGGTTTCTATGGCGAAGAAGAGCGTCGGAGATCTGACCTCAGCTGATTTGAAGGAGAAG AATGTTTACGTG |
| GenBank Accession | CT026406 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:4063283 - go to primer design |
| BLAST e Value | 8e-132 |
| Hit Clone Code (BAC ID) | MBK21 |
| Hit Gene Code | At3g12780 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | phosphoglycerate kinase 1 |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 908E11 |
| Other FSTs Supporting this Hit | CT026405 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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