Genotyping and primer details


Genotyping and primer details for 537H06 - At4g25110
Line ID 537H06
Locus At4g25110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12889544 of line 537H06 Chr4:12889544
   
Insertion allele amplicon 8474 - VL61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer VL61: 5'-ATTGAATTCGTACCTGTCCATTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12889050
   
wt amplicon (over InsPos) 06cx - VL61
wt amplicon start Chr4:12889050
wt amplicon end Chr4:12889991
wt amplicon size 942 bp
Locus specific primer VL61: 5'-ATTGAATTCGTACCTGTCCATTCT-3'
2nd locus specific primer 06cx: 5'-GAGAAAAGAAAAGGAGTTTTGGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11