Genotyping and primer details


Genotyping and primer details for 085F04 - At1g76580
Line ID 085F04
Locus At1g76580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28736337 of line 085F04 Chr1:28736337
   
Insertion allele amplicon 8474 - PH82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PH82: 5'-TCCATCTCAGTATTCAGATTCACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28735882
   
wt amplicon (over InsPos) 01qy - PH82
wt amplicon start Chr1:28735882
wt amplicon end Chr1:28736670
wt amplicon size 789 bp
Locus specific primer PH82: 5'-TCCATCTCAGTATTCAGATTCACG-3'
2nd locus specific primer 01qy: 5'-AACCAGTCATTCAACTATTCACCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11