Genotyping and primer details


Genotyping and primer details for 323E06 - At1g54210
Line ID 323E06
Locus At1g54210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20241588 of line 323E06 Chr1:20241588
   
Insertion allele amplicon 8474 - 43ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 43ra: 5'-GCGAGAAAGCACTATTCACATAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20241833
   
wt amplicon (over InsPos) 14rt - 43ra
wt amplicon start Chr1:20241251
wt amplicon end Chr1:20241833
wt amplicon size 583 bp
Locus specific primer 43ra: 5'-GCGAGAAAGCACTATTCACATAGA-3'
2nd locus specific primer 14rt: 5'-TTCTTCATTCAGTTACGAAAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11