Genotyping and primer details


Genotyping and primer details for 393A05 - At2g15830
Line ID 393A05
Locus At2g15830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:6894047 of line 393A05 Chr2:6894047
   
Insertion allele amplicon 8474 - CM11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM11: 5'-GGACTACGGTCTGGAAAACAAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6893445
   
Insertion allele amplicon 8474 - CM12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CM12: 5'-CGTCTTATTCGTCAACCTCTTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:6894747
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11