Genotyping and primer details


Genotyping and primer details for 773C12 - At2g34340
Line ID 773C12
Locus At2g34340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14489074 of line 773C12 Chr2:14489074
   
Insertion allele amplicon 8474 - 04ux
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04ux: 5'-TGTCTCTCACAATCAATGACAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14488634
   
wt amplicon (over InsPos) 54vy - 04ux
wt amplicon start Chr2:14488634
wt amplicon end Chr2:14489815
wt amplicon size 1182 bp
Locus specific primer 04ux: 5'-TGTCTCTCACAATCAATGACAACA-3'
2nd locus specific primer 54vy: 5'-TGCGTTACTCTCGGACACATATAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11