Genotyping and primer details


Genotyping and primer details for 005G04 - At1g03260
Line ID 005G04
Locus At1g03260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:796846 of line 005G04 Chr1:796846
   
Insertion allele amplicon 8474 - 01by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01by: 5'-CAAGCTCATCTACAATCATTTCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:796473
   
wt amplicon (over InsPos) 10dd - 01by
wt amplicon start Chr1:796473
wt amplicon end Chr1:797151
wt amplicon size 679 bp
Locus specific primer 01by: 5'-CAAGCTCATCTACAATCATTTCCA-3'
2nd locus specific primer 10dd: 5'-TTTTGTCTTTCTGGTATCATGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11