Genotyping and primer details


Genotyping and primer details for 387H07 - At1g18160
Line ID 387H07
Locus At1g18160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6249172 of line 387H07 Chr1:6249172
   
Insertion allele amplicon 8474 - NK86
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NK86: 5'-AGGATCCTCCCTAGCACTTAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6249515
   
wt amplicon (over InsPos) 17vg - NK86
wt amplicon start Chr1:6248624
wt amplicon end Chr1:6249515
wt amplicon size 892 bp
Locus specific primer NK86: 5'-AGGATCCTCCCTAGCACTTAACTC-3'
2nd locus specific primer 17vg: 5'-TCAAAAATCCACTCTTCATCCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11