Genotyping and primer details


Genotyping and primer details for 214G02 - At1g20900
Line ID 214G02
Locus At1g20900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7272883 of line 214G02 Chr1:7272883
   
Insertion allele amplicon 8474 - KT65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KT65: 5'-TTTTGTCGGCATTTGTGTATATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7272393
   
Insertion allele amplicon 8474 - KT66
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KT66: 5'-CACTCTCAACTATGTCAGCTCCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7273411
   
wt amplicon (over InsPos) 42rd - KT65
wt amplicon start Chr1:7272393
wt amplicon end Chr1:7273216
wt amplicon size 824 bp
Locus specific primer KT65: 5'-TTTTGTCGGCATTTGTGTATATGT-3'
2nd locus specific primer 42rd: 5'-TGTTTGAATGGTCAGATTCTCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11