Genotyping and primer details


Genotyping and primer details for 570G10 - At1g30400
Line ID 570G10
Locus At1g30400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10733651 of line 570G10 Chr1:10733651
   
Insertion allele amplicon 8474 - 26fp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26fp: 5'-AGAAATGGATACTTCTCCTGGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10733072
   
wt amplicon (over InsPos) 15gf - 26fp
wt amplicon start Chr1:10733072
wt amplicon end Chr1:10734027
wt amplicon size 956 bp
Locus specific primer 26fp: 5'-AGAAATGGATACTTCTCCTGGGAT-3'
2nd locus specific primer 15gf: 5'-CTATGGCTAGTTTACCTGCTGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11