Genotyping and primer details


Genotyping and primer details for 674B07 - At1g36160
Line ID 674B07
Locus At1g36160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:13538775 of line 674B07 Chr1:13538775
   
Insertion allele amplicon 8474 - GE38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE38: 5'-TGCTGGCTATGAGCATAAAGTAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13538251
   
Insertion allele amplicon 8474 - GE39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE39: 5'-GAAAAACCTGGTCTGAGAAAGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:13539117
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11