Genotyping and primer details


Genotyping and primer details for 791F02 - At1g44910
Line ID 791F02
Locus At1g44910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16982074 of line 791F02 Chr1:16982074
   
Insertion allele amplicon 8474 - BH67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH67: 5'-GATTCTTCCCGTTTACTCCTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16982439
   
wt amplicon (over InsPos) 06ah - BH67
wt amplicon start Chr1:16981662
wt amplicon end Chr1:16982439
wt amplicon size 778 bp
Locus specific primer BH67: 5'-GATTCTTCCCGTTTACTCCTCTCT-3'
2nd locus specific primer 06ah: 5'-TTAATCAACAGAAACGGCTATGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11