Genotyping and primer details


Genotyping and primer details for 276C07 - At1g48635
Line ID 276C07
Locus At1g48635 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17984483 of line 276C07 Chr1:17984483
   
Insertion allele amplicon 8474 - 14st
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14st: 5'-ATCTCTGGGTTCGACTTCAGTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17983914
   
wt amplicon (over InsPos) 17ue - 14st
wt amplicon start Chr1:17983914
wt amplicon end Chr1:17985011
wt amplicon size 1098 bp
Locus specific primer 14st: 5'-ATCTCTGGGTTCGACTTCAGTAAA-3'
2nd locus specific primer 17ue: 5'-CGTTATCGCAATTAAACACAGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11