Genotyping and primer details


Genotyping and primer details for 314F08 - At1g51560
Line ID 314F08
Locus At1g51560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19120408 of line 314F08 Chr1:19120408
   
Insertion allele amplicon 8474 - 42ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42ra: 5'-CACTCGGAATATCTTAGCTGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19120927
   
wt amplicon (over InsPos) 01rw - 42ra
wt amplicon start Chr1:19120038
wt amplicon end Chr1:19120927
wt amplicon size 890 bp
Locus specific primer 42ra: 5'-CACTCGGAATATCTTAGCTGAACC-3'
2nd locus specific primer 01rw: 5'-CACACAAACAAACTCAGAGAGAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11