Genotyping and primer details


Genotyping and primer details for 182C12 - At1g58250
Line ID 182C12
Locus At1g58250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21598376 of line 182C12 Chr1:21598376
   
Insertion allele amplicon 8474 - 32ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32ra: 5'-GCATAACATACTGTCCGCTCTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21597868
   
wt amplicon (over InsPos) 47si - 32ra
wt amplicon start Chr1:21597868
wt amplicon end Chr1:21598810
wt amplicon size 943 bp
Locus specific primer 32ra: 5'-GCATAACATACTGTCCGCTCTACA-3'
2nd locus specific primer 47si: 5'-GGAGAGTACACGTCTTGATGTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11