Genotyping and primer details


Genotyping and primer details for 641F12 - At1g62660
Line ID 641F12
Locus At1g62660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23202995 of line 641F12 Chr1:23202995
   
Insertion allele amplicon 8474 - 20vd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20vd: 5'-TGGTCTTCTGTTCAGGTACCTTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23202518
   
wt amplicon (over InsPos) 73vo - 20vd
wt amplicon start Chr1:23202518
wt amplicon end Chr1:23203171
wt amplicon size 654 bp
Locus specific primer 20vd: 5'-TGGTCTTCTGTTCAGGTACCTTTT-3'
2nd locus specific primer 73vo: 5'-TACGAAGCTTCCATAGATTGGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11