Genotyping and primer details


Genotyping and primer details for 138D05 - At1g69190
Line ID 138D05
Locus At1g69190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26013209 of line 138D05 Chr1:26013209
   
Insertion allele amplicon 8474 - BU21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BU21: 5'-TAGACGAGAACATGCATAAGGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26013651
   
Insertion allele amplicon 8474 - CB41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CB41: 5'-TGTCATAAATTTGATTATGCCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26012751
   
wt amplicon (over InsPos) CB41 - BU21
wt amplicon start Chr1:26012751
wt amplicon end Chr1:26013651
wt amplicon size 901 bp
Locus specific primer BU21: 5'-TAGACGAGAACATGCATAAGGTTG-3'
2nd locus specific primer CB41: 5'-TGTCATAAATTTGATTATGCCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11