Genotyping and primer details


Genotyping and primer details for 940H02 - At1g72970
Line ID 940H02
Locus At1g72970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27453095 of line 940H02 Chr1:27453095
   
Insertion allele amplicon 8474 - 36wl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 36wl: 5'-AAAATTATACTTACGCAGCATCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27453734
   
Insertion allele amplicon 8474 - AJ09
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ09: 5'-ACAGAATGGGACACACCTAAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27452486
   
Insertion allele amplicon 8474 - AJ10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AJ10: 5'-GCTTGCTTTATCGATGAATGTGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27453388
   
wt amplicon (over InsPos) AJ10 - AJ09
wt amplicon start Chr1:27452486
wt amplicon end Chr1:27453388
wt amplicon size 903 bp
Locus specific primer AJ09: 5'-ACAGAATGGGACACACCTAAAATC-3'
2nd locus specific primer AJ10: 5'-GCTTGCTTTATCGATGAATGTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11