Genotyping and primer details


Genotyping and primer details for 941B09 - At1g74720
Line ID 941B09
Locus At1g74720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28076178 of line 941B09 Chr1:28076178
   
Insertion allele amplicon 8474 - KU83
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU83: 5'-ACCGTCACTCTCAAGTACCAAAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28076680
   
Insertion allele amplicon 8474 - KU84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KU84: 5'-TGTAATGCAAGGTCCAAATGATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28075892
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11