Genotyping and primer details


Genotyping and primer details for 731C01 - At1g76850
Line ID 731C01
Locus At1g76850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28848829 of line 731C01 Chr1:28848829
   
Insertion allele amplicon 8474 - DN31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN31: 5'-AGGGAAATAGGTGGTTCTAGTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28848298
   
wt amplicon (over InsPos) 07cg - DN31
wt amplicon start Chr1:28848298
wt amplicon end Chr1:28849321
wt amplicon size 1024 bp
Locus specific primer DN31: 5'-AGGGAAATAGGTGGTTCTAGTGGT-3'
2nd locus specific primer 07cg: 5'-CGACCCTTTAAGTCACTTTTCAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11