Genotyping and primer details


Genotyping and primer details for 650H12 - At2g05420
Line ID 650H12
Locus At2g05420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1984366 of line 650H12 Chr2:1984366
   
Insertion allele amplicon 8474 - 15yp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15yp: 5'-TCTTTCTCTCTACCTCACTCAGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1983700
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11