Genotyping and primer details


Genotyping and primer details for 068A09 - At2g13620
Line ID 068A09
Locus At2g13620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:5679901 of line 068A09 Chr2:5679901
   
Insertion allele amplicon 8474 - FP08
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FP08: 5'-CCTTTACAAGCCAGTGAAGAAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:5679445
   
Insertion allele amplicon 8474 - KM38
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KM38: 5'-ACAGTAGCTGCAAAGTCTGATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:5680497
   
wt amplicon (over InsPos) KM38 - FP08
wt amplicon start Chr2:5679445
wt amplicon end Chr2:5680497
wt amplicon size 1053 bp
Locus specific primer FP08: 5'-CCTTTACAAGCCAGTGAAGAAATC-3'
2nd locus specific primer KM38: 5'-ACAGTAGCTGCAAAGTCTGATGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11