Genotyping and primer details


Genotyping and primer details for 324C12 - At2g18970
Line ID 324C12
Locus At2g18970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8228825 of line 324C12 Chr2:8228825
   
Insertion allele amplicon 8474 - 27ns
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27ns: 5'-AGGAGATTTTGAGTGAGATTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8229138
   
wt amplicon (over InsPos) 37sq - 27ns
wt amplicon start Chr2:8228512
wt amplicon end Chr2:8229138
wt amplicon size 627 bp
Locus specific primer 27ns: 5'-AGGAGATTTTGAGTGAGATTGGAA-3'
2nd locus specific primer 37sq: 5'-TCAATTCAAAGCAGTGACAAATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11