Genotyping and primer details


Genotyping and primer details for 137C10 - At2g24600
Line ID 137C10
Locus At2g24600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10454306 of line 137C10 Chr2:10454306
   
Insertion allele amplicon 8474 - CN15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CN15: 5'-AGTAGATCCACTTGATAAGGCGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10453980
   
wt amplicon (over InsPos) 14tx - CN15
wt amplicon start Chr2:10453980
wt amplicon end Chr2:10454790
wt amplicon size 811 bp
Locus specific primer CN15: 5'-AGTAGATCCACTTGATAAGGCGAA-3'
2nd locus specific primer 14tx: 5'-TGTGCTTGAGACTGACATTAGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11