Genotyping and primer details


Genotyping and primer details for 785G05 - At2g27150
Line ID 785G05
Locus At2g27150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11604104 of line 785G05 Chr2:11604104
   
Insertion allele amplicon 8474 - 15ay
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15ay: 5'-ACCAAGTTTCAAACACACAAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11604670
   
wt amplicon (over InsPos) 05jf - 15ay
wt amplicon start Chr2:11603601
wt amplicon end Chr2:11604670
wt amplicon size 1070 bp
Locus specific primer 15ay: 5'-ACCAAGTTTCAAACACACAAAATG-3'
2nd locus specific primer 05jf: 5'-GAAGTGGAGACTTTCTTGACAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11