Genotyping and primer details


Genotyping and primer details for 734G11 - At2g40815
Line ID 734G11
Locus At2g40815 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17035618 of line 734G11 Chr2:17035618
   
Insertion allele amplicon 8474 - BE12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BE12: 5'-CTTGAAGGTAAGTCGATTCCTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17034782
   
wt amplicon (over InsPos) 01bj - BE12
wt amplicon start Chr2:17034782
wt amplicon end Chr2:17035746
wt amplicon size 965 bp
Locus specific primer BE12: 5'-CTTGAAGGTAAGTCGATTCCTGAC-3'
2nd locus specific primer 01bj: 5'-CTGGAAGCTATCAGCTTAGGAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11