Genotyping and primer details


Genotyping and primer details for 957C09 - At2g40930
Line ID 957C09
Locus At2g40930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17079723 of line 957C09 Chr2:17079723
   
Insertion allele amplicon 8474 - 02bg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02bg: 5'-TATAACCAAATGCAAGCCTAATCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17079126
   
Insertion allele amplicon 8474 - 03ae
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03ae: 5'-ATAACACATACAGTAGCACTGGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17080201
   
wt amplicon (over InsPos) 02bg - 03ae
wt amplicon start Chr2:17079126
wt amplicon end Chr2:17080201
wt amplicon size 1076 bp
Locus specific primer 03ae: 5'-ATAACACATACAGTAGCACTGGCG-3'
2nd locus specific primer 02bg: 5'-TATAACCAAATGCAAGCCTAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11