Genotyping and primer details


Genotyping and primer details for 939F02 - At2g44540
Line ID 939F02
Locus At2g44540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18387585 of line 939F02 Chr2:18387585
   
Insertion allele amplicon 3144 - 39xw
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 39xw: 5'-TTTCCATTGCTCCCTACTTTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18387017
   
Insertion allele amplicon 8474 - 51xw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51xw: 5'-GTGAATACATACATAGACATAGACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18388209
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11