Genotyping and primer details


Genotyping and primer details for 094F06 - At2g45590
Line ID 094F06
Locus At2g45590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18787788 of line 094F06 Chr2:18787788
   
Insertion allele amplicon 8474 - 06qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06qz: 5'-AAACCAAGAGTCAATGCTTGAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18788143
   
wt amplicon (over InsPos) 05rp - 06qz
wt amplicon start Chr2:18787511
wt amplicon end Chr2:18788143
wt amplicon size 633 bp
Locus specific primer 06qz: 5'-AAACCAAGAGTCAATGCTTGAGAT-3'
2nd locus specific primer 05rp: 5'-AAGAGCAAGGATGTTGTTGAAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11