Genotyping and primer details


Genotyping and primer details for 283A09 - At2g47240
Line ID 283A09
Locus At2g47240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19395733 of line 283A09 Chr2:19395733
   
Insertion allele amplicon 8474 - 79cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 79cs: 5'-ATATAAAATGACAGGCAACCCAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19395230
   
wt amplicon (over InsPos) 02dn - 79cs
wt amplicon start Chr2:19395230
wt amplicon end Chr2:19396116
wt amplicon size 887 bp
Locus specific primer 79cs: 5'-ATATAAAATGACAGGCAACCCAAC-3'
2nd locus specific primer 02dn: 5'-AACAGAGCAAAAACGATGAAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11