Genotyping and primer details


Genotyping and primer details for 015E12 - At3g03250
Line ID 015E12
Locus At3g03250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:749980 of line 015E12 Chr3:749980
   
Insertion allele amplicon 8474 - KC79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KC79: 5'-AAAGCTAGAACTAACCCCTCGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:750483
   
wt amplicon (over InsPos) 03dw - KC79
wt amplicon start Chr3:749613
wt amplicon end Chr3:750483
wt amplicon size 871 bp
Locus specific primer KC79: 5'-AAAGCTAGAACTAACCCCTCGAAC-3'
2nd locus specific primer 03dw: 5'-GCTAAGCTGCAAAAAGAAAAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11