Genotyping and primer details


Genotyping and primer details for 695F05 - At3g04790
Line ID 695F05
Locus At3g04790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1313325 of line 695F05 Chr3:1313325
   
Insertion allele amplicon 8474 - 16di
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16di: 5'-ATAACAAACCTGCTTGAGAGCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1312701
   
wt amplicon (over InsPos) 66dy - 16di
wt amplicon start Chr3:1312701
wt amplicon end Chr3:1313874
wt amplicon size 1174 bp
Locus specific primer 16di: 5'-ATAACAAACCTGCTTGAGAGCATT-3'
2nd locus specific primer 66dy: 5'-TGTAACGAGTTTGGTATCATCAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11