Genotyping and primer details


Genotyping and primer details for 184C08 - At3g08040
Line ID 184C08
Locus At3g08040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2566670 of line 184C08 Chr3:2566670
   
Insertion allele amplicon 8474 - 15cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15cy: 5'-CCGGATATCTTTGAACCATCTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2566350
   
wt amplicon (over InsPos) 24dy - 15cy
wt amplicon start Chr3:2566350
wt amplicon end Chr3:2567289
wt amplicon size 940 bp
Locus specific primer 15cy: 5'-CCGGATATCTTTGAACCATCTAGT-3'
2nd locus specific primer 24dy: 5'-AAACAGAAATGAATTTGATACGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11