Genotyping and primer details


Genotyping and primer details for 675G06 - At3g08950
Line ID 675G06
Locus At3g08950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2729571 of line 675G06 Chr3:2729571
   
Insertion allele amplicon 8474 - 21fx
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21fx: 5'-TGGTGGTTGGAGTTTTGATATGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2729139
   
wt amplicon (over InsPos) 07gv - 21fx
wt amplicon start Chr3:2729139
wt amplicon end Chr3:2729830
wt amplicon size 692 bp
Locus specific primer 21fx: 5'-TGGTGGTTGGAGTTTTGATATGTA-3'
2nd locus specific primer 07gv: 5'-CAGGTCGGAGTTTTTGTCTCTATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11